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Probing DNA recognition and catalysis in a DNA glycosylase super-family

Reference	B11484
Principal Investigator / Supervisor	Dr Renos Savva
Co-Investigators / Co-Supervisors	Professor Geoffrey Baldwin, Professor Laurence Pearl
Institution	Birkbeck College
Department	Biological Sciences
Funding type	Research
Value (£)	162,399
Status	Completed
Type	Research Grant
Start date	01/11/1999
End date	01/11/2002
Duration	36 months

Abstract

There are two distinct classes of enzyme that are responsible for the removal of uracil from DNA; the uracil-DNA glycosylases (UDG) and the uracil/thymine:guanine mismatch-DNA glycosylases (MUG/TDG). Structural studies have revealed a remarkable similarity of form and function, despite clear biochemical differences, which may be explained by a number of key amino acid substitutions at the active sites of the enzymes. As a result, there exists two distinct mechanisms for the recognition and removal of the DNA lesion. We intend to elucidate these mechanisms by using a multidisciplinary approach to the study of UDG and MUG. This will involve mutagenesis to switch the active site residues of each enzyme and analysis of the mutants using single turnover kinetics alongside structural studies of the mutants, both alone and in enzyme-DNA complexes.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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