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In vitro studies of type II polyketide synthases

Reference	B11480
Principal Investigator / Supervisor	Professor Thomas Simpson
Co-Investigators / Co-Supervisors	Professor Russell Cox, Dr John Crosby
Institution	University of Bristol
Department	Chemistry
Funding type	Research
Value (£)	364,722
Status	Completed
Type	Research Grant
Start date	01/12/1999
End date	01/02/2003
Duration	38 months

Abstract

The precise mechanisms of chain assembly, cyclisation and further modifications catalysed by Type II polyketide synthases will be studied using in vitro systems reconstituted from purified PKS components. ACPs acylated with synthetic assembly intermediates and analogues will be used to probe the mechanisms and to produce novel compounds. Mutant ACPs will be used to probe the role of specific residues in stabilisation, folding and cyclisation of PK intermediates. Interactions of PKS and FAS components of ACPS and MCAT will be investigated. The overall aim is to understand how existing biosynthetic pathways can be manipulated to allow production of novel bioactive compounds.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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