Award details

Molecular analysis of receptor-toxin recognition and cytolytic pore formation in insect midgut cell membranes and supported lipid layers

Reference	B11458
Principal Investigator / Supervisor	Professor David Ellar
Co-Investigators / Co-Supervisors
Institution	University of Cambridge
Department	Biochemistry
Funding type	Research
Value (£)	184,741
Status	Completed
Type	Research Grant
Start date	01/12/1999
End date	11/02/2004
Duration	50 months

Abstract

The proposal seeks fundamental data on the molecular bass of toxicity and specificity of the insecticidal Bacillus thuringiensis gamma-endotoxins. It is proposed to: (1) Complete the characterisation of toxin binding site(s) on an insect midgut epithelial aminopeptidase-N- receptor; (2) redefine a novel SPR system for studying toxin binding/insertion with the receptor incorporated in a lipid bilayer; (3) identify and characterise the membrane receptor and lytic events in a newly discovered second midgut cell lytic mechanism triggered by these toxins. (The existence of the latter may be linked to our recent finding that insecticidal specificity of the toxins may be determined) by two lectin-like domains acting in concert or independently.); and (4) complete the X-ray crystallographic analysis of a complex between a Bacillus thuringiensis gamma-endotoxin and a receptor determinant.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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