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Isolation of ovarian GnSAF/IF from granulosa cell-conditioned medium

Reference	B11456
Principal Investigator / Supervisor	Professor Paul Fowler
Co-Investigators / Co-Supervisors	Dr William Harrison
Institution	University of Aberdeen
Department	Department of Obstetrics & Gynaecology
Funding type	Research
Value (£)	98,400
Status	Completed
Type	Research Grant
Start date	01/06/1999
End date	01/06/2001
Duration	24 months

Abstract

Gonadotrophin surge attenuating/inhibiting factor (GnSAF/IF), an ovarian hormone which regulates gonadotrophin secretion, remains to be conclusively characterised. This study will isolate GnSAF/IF from granulosa cell culture- conditioned medium rather than follicular fluid to minimise numbers of contaminating proteins. AIMS: i. To generate N-terminal amino acid sequences and construct synthetic peptides for GnSAF/IF. ii. To generate antibodies to purify GnSAF/IF and screen ovarian granulosa cell cDNA libraries. iii. To confirm the specificity of synthetic peptides and antisera, using GnSAF/IF bioassay and immunoaffinity techniques and subtractive antibody screening. This focused project will provide information for a major research programme, including studies into in vivo effects of GnSAF/IF, production of recombinant GnSAF/IF and development of GnSAF/IF immunoassays.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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