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Atomic force microscopy of binding dynamics of DNA restriction-modification enzymes

Reference	B11179
Principal Investigator / Supervisor	Dr Robert Henderson
Co-Investigators / Co-Supervisors	Professor John Edwardson
Institution	University of Cambridge
Department	Pharmacology
Funding type	Research
Value (£)	66,788
Status	Completed
Type	Research Grant
Start date	01/01/1999
End date	01/01/2002
Duration	36 months

Abstract

We will use atomic force microscopy (AFM) to analyse the mechanisms of DNA binding, translocation and cleavage by the prokaryotic restriction-modification enzyme EcoKI, and other enzymes that have a similar mode of action. The application of AFM will allow us to study the dynamics of the enzyme-DNA interactions in real time, an approach that has already provided us with new insights into the way in which EcoKI works. Specifically, we will study the properties of both wild-type EcoKI and a selection of mutants that have modifications in the subunits responsible for sequence recognition, methylation and DNA restriction. In this way we will dissect these distinct functions of the enzyme, and generate an overall view of its mechanism of action.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	BioImaging (BI) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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