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X-ray structure of succinyl-CoA transferase and the structural basis for half site reactivity

ReferenceB10932
Principal Investigator / Supervisor Professor Peter Shoolingin-Jordan
Co-Investigators /
Co-Supervisors
Institution University of Southampton
DepartmentCentre for Biological Sciences
Funding typeResearch
Value (£) 188,620
StatusCompleted
TypeResearch Grant
Start date 11/01/1999
End date 10/06/2002
Duration42 months

Abstract

X-ray structure determination of succinyl-CoA transferase from mammalian (porcine) heart mitochondria. Refinement of the structure to 1.5 angstrom. X-ray structure determination of the catalytic enzyme-CoA intermediate and correlation of the structure with the newly- discovered properties of half site reactivity. Identification of the catalytic groups and the structural basis for substrate recognition by a combination of X-ray analyses, site-directed mutagenesis and comparative studies between the glutaconyl-CoA transferase from Acidaminococcus fermentens and other transferases.

Summary

unavailable
Committee Closed Committee - Biomolecular Sciences (BMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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