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Binding of C2 domains at phospholipid-bilayer surfaces

Reference	B10896
Principal Investigator / Supervisor	Professor Anthony Lee
Co-Investigators / Co-Supervisors	Professor J East
Institution	University of Southampton
Department	Centre for Biological Sciences
Funding type	Research
Value (£)	162,572
Status	Completed
Type	Research Grant
Start date	01/04/1999
End date	01/04/2002
Duration	36 months

Abstract

We will study the interaction of C2 domains of protein kinase C and phospholipase A (cPLA2) with lipid bilayers. We will produce recombinant C2 domains, introducing Trp residues at chosen positions for fluorescence studies. We will determine the bound orientation of the domains and determine the energetics and specificity of binding to anionic lipids in the membrane, distinguishing between non-specific (electrostatic) and structurally- specific binding. We will determine residues in the C2 domain important for interaction with the lipid bilayer.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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