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Engineering cytochrome p450cam into an alkane hydroxylase
Reference
B10666
Principal Investigator / Supervisor
Professor Luet Wong
Co-Investigators /
Co-Supervisors
Institution
University of Oxford
Department
Oxford Chemistry
Funding type
Research
Value (£)
153,981
Status
Completed
Type
Research Grant
Start date
01/08/1999
End date
01/08/2002
Duration
36 months
Abstract
One of the most intriguing questions in chemistry and biochemistry is that, whilst the cytochrome P450 superfamily of monooxygenases can catalyse the oxidation of an extensive range of compounds, including alkanes such as hexane, no P450 enzyme capable of oxidising methane has been isolated. The main objective of the proposed project is to investigate whether the ferryl species, which is the proposed active intermediate in C-H bond oxidation by P450 enzymes, can oxidise the C-H bond of methane. The approach is to engineer a well- characterised P450 enzyme, P450cam such that its active site can bind methane in close proximity to the haem. The engineering of P450cam into a haem methane monooxygenase is a very exciting prospect, and preliminary results indicate that P450cam can be engineered to show significant NADH turnover activity (200 min) with propane as substrate.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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