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Characterisation of E. coli respiratory enzymes by protein film voltammetry
Reference
B10492
Principal Investigator / Supervisor
Dr Stephen Attwood
Co-Investigators /
Co-Supervisors
Institution
University of Oxford
Department
Oxford Chemistry
Funding type
Research
Value (£)
147,535
Status
Completed
Type
Research Grant
Start date
01/05/1999
End date
01/05/2002
Duration
36 months
Abstract
A novel technique, protein film voltammetry (PFV), will be used to obtain a quantitative, integrated profile of the redox centres and catalytic action of two complex multi-centred electron-transport (ET) enzymes. Fumarate reductase (FAD, 3 iron-sulfur clusters) and DMSO reductase (molybdopterin, 4 iron-sulphur clusters) are membrane respiratory enzymes induced in E. coli under anaerobiosis, and represent important model systems for biological electron transfer. PFV will be used to interrogate and make in-depth comparisons of genetically engineered variants, simultaneously revealing individual active sites, their reduction potentials (including rapid-cycle time-resolved measurements), the kinetics and energetics of electron transfer and coupled reactions, and intramolecular relays and feedback effects, each of which are key factors defining the action of ET enzymes.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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