Award details

Synthesis and study of macromolecular assemblies

Reference	B10450
Principal Investigator / Supervisor	Professor Chris Abell
Co-Investigators / Co-Supervisors	Professor Ben Luisi
Institution	University of Cambridge
Department	Chemistry
Funding type	Research
Value (£)	156,780
Status	Completed
Type	Research Grant
Start date	01/04/1999
End date	01/07/2003
Duration	51 months

Abstract

1) dsDNA will be used as a molecular scaffold on which to assemble proteins spatially in macromolecular constructs (Mr>200 000). Two discrete strategies for covalently attaching the proteins are described. Preliminary results, including atomic force microscopy images (AFM) of DNA 'dumbbells' are presented. Multienzyme constructs will be assembled and studied. 2) AFM will be used to study the assembly of two transcription regulatory complexes, and higher order assemblies. 3) The DNA- protein complexes from 1) will be used to modify or study the complexes in 2), and study the steric demands of some DNA modifying enzymes. The use of these constructs, coupled with analysis by AFM, allows completely new questions to be asked.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search