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Towards a mechanistic rationalisation of the extradiol and intradiol catechol dioxygenases
Reference
B10351
Principal Investigator / Supervisor
Professor Timothy Bugg
Co-Investigators /
Co-Supervisors
Professor G Reid
Institution
University of Warwick
Department
Chemistry
Funding type
Research
Value (£)
158,516
Status
Completed
Type
Research Grant
Start date
26/03/1999
End date
26/03/2002
Duration
36 months
Abstract
A mechanistic hypothesis is presented for the extradiol and intradiol catechol dioxygenases, in which the number of available iron co-ordination sites dictates the conformation of a bound hydroperoxide intermediate, and hence the choice of reaction pathway. The project will involve: 1) preparation of carba-analogues as mechanistic probes for C-1 vs. C-2 peroxy intermediates in the reaction catalysed by 2,3- dihydroxyphenylpropionate 1,2-dioxygenase (MhpB) from E. coli; 2) monitoring of dioxygenase-catalysed rearrangements of the carba- analogues; 3) use of enantiomerically pure carba- analogues to study the absolute stereochemistry of catechol dioxygenase catalysis; 4) use of synthetic transition metal complexes to test the mechanistic hypothesis.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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