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RNA folding and catalytic activity: the hairpin ribozyme
Reference
B10186
Principal Investigator / Supervisor
Professor David Lilley
Co-Investigators /
Co-Supervisors
Institution
University of Dundee
Department
College of Life Sciences
Funding type
Research
Value (£)
172,808
Status
Completed
Type
Research Grant
Start date
01/05/1999
End date
01/05/2002
Duration
36 months
Abstract
RNA catalysis is of both fundamental importance and practical significance. The purpose of this project is to relate the origin of catalytic activity in the hairpin ribozyme to the folding of the RNA. The natural form of this ribozyme in the viral RNA is that of a four-way junction, and we have shown that the activity of the ribozyme is modulated by the conformation of the junction. We will use fluorescence resonance energy transfer to study the folding of the ribozyme and it's kinetics. We will study the structure of the folded ribozyme in detail, and the location and role of structural metal ions. This provides an excellent opportunity to study structure- function relationships in RNA catalysis.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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