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Structure determination of nucleic acid motifs using a defined supramolecular scaffold

Reference	B09849
Principal Investigator / Supervisor	Professor Simon Phillips
Co-Investigators / Co-Supervisors	Professor Peter Stockley
Institution	University of Leeds
Department	Inst of Molecular & Cellular Biology
Funding type	Research
Value (£)	326,920
Status	Completed
Type	Research Grant
Start date	01/10/1998
End date	01/04/2002
Duration	42 months

Abstract

We intend to take advantage of a unique opportunity presented by the bacteriophage MS2 operator capsid system. We will use it as a molecular support to hold a wide range of small nucleic acid motifs in a crystal lattice, and render them amenable to high resolution X-ray crystallographic structure determination without having to rely on the uncertain step of crystallising them independently. This will enable us to determine structures reliably and rapidly for biologically important RNA and DNA motifs, such as rRNA recognition motifs, the HIV TAR/RRE sites and their complexes with small molecules. In addition, we will determine the structures of a series of complexes between the phage coat protein-and selected RNA aptamers, which will reveal the general principles of sequence-specific RNA-protein recognition.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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