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Structural biology of ModE a molybdate-dependent transcriptional regulator

ReferenceB09838
Principal Investigator / Supervisor Professor William Hunter
Co-Investigators /
Co-Supervisors
Institution University of Dundee
DepartmentCollege of Life Sciences
Funding typeResearch
Value (£) 137,118
StatusCompleted
TypeResearch Grant
Start date 01/08/1998
End date 01/12/2002
Duration52 months

Abstract

Molybdenum is an essential element and the control of Mo uptake, the signalling mechanism, the regulation of gene expression and hence metabolism is carried out by a molybdate dependent transcriptional regulator called ModE. This protein can be classified as a member of the LysR family of transcriptional regulators. Crystals of E. coli ModE have been obtained that are suitable for a crystallographic analysis. Support is requested to allow for the characterisation of distinct conformational states of ModE and to attempt co-crystallisation of ModE to the DNA sequence to which it binds. The results could be the first complete picture of a member of this family of transcriptional regulators (repressor and activating stages) and an understanding of the structural biology implicated in the regulation of Mo metabolism.

Summary

unavailable
Committee Closed Committee - Biomolecular Sciences (BMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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