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Recognition in flavocytochrome b2: natural and forced evolution

Reference	B09837
Principal Investigator / Supervisor	Professor Stephen Chapman
Co-Investigators / Co-Supervisors	Professor Graeme Reid
Institution	University of Edinburgh
Department	Sch of Chemistry
Funding type	Research
Value (£)	167,776
Status	Completed
Type	Research Grant
Start date	01/01/1999
End date	01/07/2002
Duration	42 months

Abstract

Flavocytochrome b2 (Fb2) is a lactate:cytochrome c oxidoreductase. Each subunit consists of two domains: one containing flavin and the other haem. The precise details of how the inter-molecular electron transfer from Fb2 to cytochrome c occurs are not well understood. This raises, question 1: Where on the surface of Fb2 is the recognition site for cytochrome c? It is known that electron transfer from flavin to cytochrome c is insignificant, hence, question 2: Why is this electron transfer negligible when the driving force for it is greater than 0.3V? A possible answer is that recognition between these proteins is virtually non-existent. With our success in expressing the flavin domain in E. coli, we are now ideally placed to answer this. In addition we can also address, question 3: Can we design and construct, de novo, an efficient recognition site for cytochrome c on the surface of the flavin domain.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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