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Lipid-induced protein folding
Reference
B09547
Principal Investigator / Supervisor
Dr Teresa Pinheiro
Co-Investigators /
Co-Supervisors
Institution
University of Warwick
Department
Biological Sciences
Funding type
Research
Value (£)
87,219
Status
Completed
Type
Research Grant
Start date
16/11/1998
End date
16/11/2000
Duration
24 months
Abstract
It is proposed to study the kinetic mechanism of lipid-induced folding of the precursor mitochondrial protein, apocytochrome c, using stopped-flow spectroscopic techniques, including fluorescence and circular dichroism. The main goal is to understand the physical roles underlying the mechanism of lipid-induced protein folding. For this aim various lipid systems, including phospholipid vesicles and micelles, will be used. A fundamental question to protein folding will be addressed: whether the lipid-induced folding mechanism of apocytochrome c follows the folding pathway described for the holoprotein in solution. For this aim variant forms of apocytochrome c will be produced using an established expression system for cytochrome c. This study will provide fundamental structural insight into the mechanism of insertion and translocation through lipid membranes, by identifying the role of such membrane-associated folding intermediates.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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