Award details

Algorithms for predicting sequence-dependent DNA structure

Reference	B09458
Principal Investigator / Supervisor	Professor Christopher Hunter
Co-Investigators / Co-Supervisors
Institution	University of Sheffield
Department	Chemistry
Funding type	Research
Value (£)	106,862
Status	Completed
Type	Research Grant
Start date	01/04/1998
End date	01/04/2001
Duration	36 months

Abstract

The aim is to develop a computational method for converting primary DNA base sequence information into three-dimensional atomic level structures, which can be used to rationalise and predict functional properties. The proposal builds on methods which we have developed for accurately predicting the structures of dinucleotide steps. We now plan to extend these methods to model much larger structures. These structures will be built up gradually from dinucleotides to tetranucleotides, to oligomers, to long stretches of hundreds of bases. At each stage the results will be evaluated against experimental data from X-ray crystal structures, gel running, protein binding and wrapping on nucleosomes. The theoretical approach will allow us to study not only regular DNA but also the effects of modifications such as methylation of cytosine which plays a key role in biology.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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