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Crystal structures of three B-helical acetyltransferases

ReferenceB09328
Principal Investigator / Supervisor Professor Peter Moody
Co-Investigators /
Co-Supervisors
Institution University of Leicester
DepartmentBiochemistry
Funding typeResearch
Value (£) 137,190
StatusCompleted
TypeResearch Grant
Start date 11/09/1998
End date 11/09/2001
Duration36 months

Abstract

NodL, SAT and XAT are three microbial O-acetyltransferases that share a common core of the recently identified trimer of left-handed beta-helices within their structures and have acetyl-CoA as the acyl donor and yet very different acyl acceptors. This core means the structures differ considerably from that of Chloramphicol Acetyl Transferase (CAT), which is also trimeric and has evolved to catalyse O-acetylation from the same donor. The determination of the crystal structures of these three enzymes will allow us to compare and contrast the structural similarities of the core of the molecule with the differing means of molecular recognition. All three enzymes have been crystallised and the structures of the enzymes with their substrates and co-factors will be determined.

Summary

unavailable
Committee Closed Committee - Biomolecular Sciences (BMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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