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The process of enzyme degradation of cellulose observed and felt at the molecules level
Reference
B08614
Principal Investigator / Supervisor
Professor Mervyn Miles
Co-Investigators /
Co-Supervisors
Institution
University of Bristol
Department
Bristol Heart Institute
Funding type
Research
Value (£)
123,018
Status
Completed
Type
Research Grant
Start date
01/10/1997
End date
31/01/2001
Duration
40 months
Abstract
Imaging of protein molecules by scanning probe microscopy (SPM) is now almost routine in our laboratory. We have achieved ultrahigh-resolution of cellulose molecules such that individual glucose residues were visualised. By bringing these imagining techniques together with the capability of SPM to operate in an aqueous environment and to follow a process in real time, we will observe, at the molecular level, the enzymic hydrolysis of cellulose by cellulases. Endo- and exo-type enzymes will be examined individually. Cellulases generally consist of: a cellulose binding (CBD) and a catalytic domain (CD) joined by a linker section. We will follow the coordinated motions of these two domains during hydrolysis. The local changes in mechanical properties will also be mapped. The conformational motion of these domains during hydrolysis will be compared to that on control surfaces.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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