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Better protein crystal production by systematic screening and controlled growth
Reference
B08498
Principal Investigator / Supervisor
Professor David Blow
Co-Investigators /
Co-Supervisors
Dr Peter Brick
,
Professor John Squire
Institution
Imperial College London
Department
Biological Sciences
Funding type
Research
Value (£)
243,417
Status
Completed
Type
Research Grant
Start date
01/11/1997
End date
01/11/2000
Duration
36 months
Abstract
Protein structure analysis is a mature science with well developed techniques, but there is ever increasing demand in the medical, pharmaceutical, chemical and agricultural industries for structures of newly purified or newly engineered proteins. Often the main barrier is the preparation of crystals of adequate quality for accurate analysis. We shall make systematic studies of protein crystallisation : to improve screening techniques to discover crystallisation conditions; to improve quality by better control of crystal nucleation and growth; and to improve crystal order (e.g. by growth in gels). Techniques will be developed using proteins already known to crystallise, and will then be applied to proteins where crystallisation difficulties have been encountered (luciferase complexes, LysU, tRNA complexes, viral proteins, collagens IV and VIII, (beta-actinin etc).
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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