Award details

The elucidation of the 3-D structure of two colbalmin biosynthetic enzymes CbiK and CbiF

Reference	B08486
Principal Investigator / Supervisor	Professor Keith Wilson
Co-Investigators / Co-Supervisors
Institution	University of York
Department	Chemistry
Funding type	Research
Value (£)	99,090
Status	Completed
Type	Research Grant
Start date	01/10/1997
End date	01/03/2001
Duration	41 months

Abstract

Only microorganisms can biosynthesise vitamin B12. The synthesis of the highly decorated corrin ring proceeds via a series of complex steps. We have obtained diffraction quality crystals of two of the enzymes: Bacillus megatarium CbiF, a AdoMet dependent C-methyltransferase, and Salmonella typhimurium CbiK, the anaerobic cobalamin cobaltochelatase, and propose to elucidate their structure. In this collaborative program between UCL London and York, we further propose to investigate the structure- function relationships of CbiF and CbiK by mutagenesis and enzymology. In addition, we already have overexpressing clones and will screen for crystallisation conditions for other purified cobalamin biosynthetic enzymes. (Joint with grant B08652).

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

B08652 The elucidation of the 3-D structure of two colbalmin biosynthetic enzymes CbiF and CbiK

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