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A structural approach to the enzymology of biotin biosynthesis

ReferenceB06790
Principal Investigator / Supervisor Professor Lindsay Sawyer
Co-Investigators /
Co-Supervisors
Professor Robert Baxter
Institution University of Edinburgh
DepartmentInst of Cell and Molecular Biology
Funding typeResearch
Value (£) 174,552
StatusCompleted
TypeResearch Grant
Start date 01/10/1996
End date 01/11/1999
Duration37 months

Abstract

The aim is to complete our successful studies of the detailed molecular mechanism of the Escherichia coli bioD gene product, dethiobiotin synthetase, using substrate analogues and site-directed mutants to examine especially product release but now to include other members of the pathway which we have now over-expressed and purified. These are the gene products bioA diaminopelargonic acid aminotransferase, bioF ketoaminopelargonic acid synthetase and bioB, biotin synthase, which is now known to require a flavodoxin/ferredoxin reductase (the fpr gene product which we have crystallised) and probably one further protein component.

Summary

unavailable
Committee Closed Committee - Biomolecular Sciences (BMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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