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Engineering of protein L for detection and preparation of Fab and Fv fragments
Reference
B06489
Principal Investigator / Supervisor
Professor Brian Sutton
Co-Investigators /
Co-Supervisors
Professor Michael Gore
Institution
King's College London
Department
GKT School of Biomedical Sciences
Funding type
Research
Value (£)
151,185
Status
Completed
Type
Research Grant
Start date
01/11/1996
End date
01/03/1999
Duration
28 months
Abstract
The overall objective is the design and production of a protein reagent for detection, preparation and purification of Fab and Fv fragments. Bacterial protein L (SpL) binds only to V kappa domains, and our aim is to modify its binding properties by mutagenesis to encompass V lambda, and to allow for elution of bound Ig fragments under mild conditions by modifying the pH dependence of binding. We have cloned and expressed SpL in E. coli and determined its crystal structure, and will crystallise complexes with Fab and Fv for X-ray analysis. Random mutagenesis by phage display will be performed, and mutants assayed by ELISA, fluorescence and BIAcore SPR.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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