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Correlated single-molecule measurements during acto-myosin force production
Reference
B06103
Principal Investigator / Supervisor
Dr Justin Molloy
Co-Investigators /
Co-Supervisors
Institution
University of York
Department
Biology
Funding type
Research
Value (£)
277,810
Status
Completed
Type
Research Grant
Start date
01/09/1996
End date
01/09/1999
Duration
36 months
Abstract
We will combine single-molecule imaging with a laser tweezers mechanical transducer to make correlated measurements during single acto-S1 interactions. To achieve this we will develop the technique of single-molecule fluorescence imaging and use two fluorescence methods to monitor myosin head (S1) orientation (by fluorescence anisotropy) and ligand binding (using a fluorescent ATP analogue) during the mechanical cycle of a single S1 molecule. Simultaneously with the fluorescence measurements we will measure and manipulate the mechanical state of the S1 molecule using optical tweezers.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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