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X-ray structural studies of the bacterial alpha- toxin and their implication in gas-gangrene
Reference
B05996
Principal Investigator / Supervisor
Professor David Moss
Co-Investigators /
Co-Supervisors
Dr Ajit Basak
Institution
Birkbeck College
Department
Biological Sciences
Funding type
Research
Value (£)
163,695
Status
Completed
Type
Research Grant
Start date
01/10/1996
End date
01/10/1999
Duration
36 months
Abstract
We propose to determine the structure of bacterial alpha-toxin of Clostridium perfringens, the toxin responsible for gas-gangrene, by X-ray analysis in three environments. The ground work (expression, purification and crystallisation) has already been carried out. Diffraction data to 1.9, 2.3 and 2.8A resolution have been obtained for three different crystal forms. We also intend to study the 3D-structure of alpha-toxin complexed with different inhibitors. We also plan to generate mutants of the alpha-toxin within the C-terminal domain and identify residues which play a role in hemolytic activity of the toxin. This work is a collaboration with CBDE Porton Down where the cloning and expression will be carried out.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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