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Characterisation and crystallisation of LH2 from Rhodopseudomonas palustris

ReferenceB04960
Principal Investigator / Supervisor Dr Miroslav Papiz
Co-Investigators /
Co-Supervisors
Dr Anna Lawless
Institution STFC - Laboratories
DepartmentPhoton Science
Funding typeResearch
Value (£) 95,117
StatusCompleted
TypeResearch Grant
Start date 05/02/1996
End date 05/02/1999
Duration36 months

Abstract

In recent months the first X-ray structure of a bacterial integral membrane LH2 from Rps. acidophila strain 10050 has been determined to 2.5 Angstroms resolution with data collected at the SRS Daresbury. We wish to extend this work to the crystal structure of a low light (LL) form of LH2 from Rps. palustris. It differs in the bacteriochlorophyll/carotenoid ratio of 3:1 compare to 2:1 of the high light (HL) form. The ratio of B800 to B850 changes from 1:2 in the HL form to 2:3 or perhaps as high as 2:1 in the LL form. The understanding of these particular pigment modifications in LL LH2 may give insight into their role in low light adaptation, and give additional information about light trapping mechanisms.

Summary

unavailable
Committee Closed Committee - Biomolecular Sciences (BMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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