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The structural basis of dual-function transcriptional regulation in the LysR family protein, CysB
Reference
B04808
Principal Investigator / Supervisor
Professor Anthony Wilkinson
Co-Investigators /
Co-Supervisors
Institution
University of York
Department
Chemistry
Funding type
Research
Value (£)
114,216
Status
Completed
Type
Research Grant
Start date
01/11/1995
End date
01/11/1998
Duration
36 months
Abstract
CysB activates transcription of the cysteine regulon. The inducer N-acetylserine enhances the affinity of CysB for the cysJ, cysK and cysP promoters, leading to activation of transcription at these loci, and simultaneously lowers its affinity for the cysB promoter which partially relieves repression at this site. We propose to grow crystals and determine structures of CysB in complex with cofactor and with DNA to examine the structural basis of the opposing effects of the inducer on CysB binding at the two types of promoter. A key element of the study will be defining appropriate sizes and sequences of the DNA ligands. These studies have wider significance as CysB belongs to the large and structurally uncharacterised LysR family of gene activator proteins.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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