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Structure specificity and mechanism of bacterial formamide hydrolases
Reference
B04802
Principal Investigator / Supervisor
Professor Laurence Pearl
Co-Investigators /
Co-Supervisors
Dr C Jones
Institution
University College London
Department
Structural Molecular Biology
Funding type
Research
Value (£)
199,819
Status
Completed
Type
Research Grant
Start date
01/01/1996
End date
01/01/1999
Duration
36 months
Abstract
Many bacteria express amide hydrolases which enable them to utilise small amides such as acetamide or formamide as sole nitrogen sources. We have now crystallised two formamide- specific amidases from Methylophilus methylotrophus and Mycobacterium smegmatis, and propose to determine the 3-dimensional structure of these in order to understand how they are able to specifically bind, and hydrolyse substrates as small as formamide. We will also investigate the essential residues in these enzymes by selection of phenotypic variants generated by molecular evolution in continuous culture.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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