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Integration of EcoRV structure with function

Reference	B04759
Principal Investigator / Supervisor	Professor Steve Halford
Co-Investigators / Co-Supervisors	Professor R Brady
Institution	University of Bristol
Department	Biochemistry
Funding type	Research
Value (£)	311,575
Status	Completed
Type	Research Grant
Start date	01/02/1996
End date	01/02/1999
Duration	36 months

Abstract

The mechanism by which the EcoRV endonuclease cleaves DNA at one particular sequence will be investigated by perturbing either the DNA-recognition functions or the catalytic processes, analysing the consequences of the perturbation by biochemical methods and then determining the structures of the resultant EcoRV-DNA complexes by X-ray crystallography. The perturbations will include: (i) mutant proteins, with single amino acid substitutions; (ii) altered oligodeoxynucleotides, with either a base, sugar or phosphate replaced by an appropriate analogue; (iii) different metal ions, by changing the catalytic cofactor, Mg2+, to another metal ion. The structural and functional studies will be fully integrated with each other.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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