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The construction of a new pathway for biological electron transfer
Reference
B04519
Principal Investigator / Supervisor
Professor Mike Jones
Co-Investigators /
Co-Supervisors
Institution
University of Sheffield
Department
Molecular Biology and Biotechnology
Funding type
Research
Value (£)
121,856
Status
Completed
Type
Research Grant
Start date
01/10/1995
End date
01/10/1998
Duration
36 months
Abstract
The principles underlying the design of an efficient pathway for biological electron transfer will be investigated using a uniquely constructive approach. A new pathway for electron transfer within the photoreaction centre of Rhodobacter sphaeroides will be constructed, using as a template the inactive branch of cofactors associated with the M subunit. The new pathway will be constructed by site-specific mutagenesis of the reaction centre protein, and the direction, rate and efficiency of ultrafast electron transfer in the mutated proteins assessed using time resolved and steady state spectroscopic techniques. The rate and efficiency of electron transfer down the new pathway will be optimised by directed mutagenesis.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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