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Regulation of NF-kappaB DNA binding activity: defining the determinants of molecular recognition

Reference	B04253
Principal Investigator / Supervisor	Professor Ronald Hay
Co-Investigators / Co-Supervisors
Institution	University of St Andrews
Department	Biology
Funding type	Research
Value (£)	159,657
Status	Completed
Type	Research Grant
Start date	01/05/1994
End date	01/05/1997
Duration	36 months

Abstract

1. Employ photochemical crosslinking, chemical protection and mutagenesis to identify amino acids in the NF-kB transcription factor that are in direct contact with the DNA binding site and evaluate their contribution to recognition. 2. Use partial proteolysis, chemical protection and mutagenesis to define how individual residues, present at the interface between NF-kB and the inhibitor protein IkB, contribute to molecular recognition. 3. Using appropriately labelled NF-kB subunits use fluorescence energy transfer measurements to establish how IkB inhibits NF-kB DNA binding activity.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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