Award details

Modulation of intra- and inter-protein electron transfer in an oxidoreductase by key interface residues

Reference	B04227
Principal Investigator / Supervisor	Professor Stephen Chapman
Co-Investigators / Co-Supervisors	Professor Graeme Reid
Institution	University of Edinburgh
Department	Sch of Chemistry
Funding type	Research
Value (£)	135,709
Status	Completed
Type	Research Grant
Start date	01/06/1994
End date	01/06/1997
Duration	36 months

Abstract

Two recent developments will allow us to investigate intra- and inter-protein electron transfer to a new level of detail, using flavocytochrome b2 as an experimental system. Firstly, the modelling of a realistic electron transfer complex between four molecules of cytochrome c and a flavocytochrome b2 tetramer presents us with a testable model for protein-protein interactions that promote complex formation. This will form the basis of the major part of the work proposed here. Secondly, the recent determination of crystal structures of mutant forms of flavocytochrome b2 demonstrates the ability to analyse structure-function correlations in fine molecular detail and we shall analyse intra-protein electron transfer in flavocytochrome b2 in relation to the structures of these two mutant structures which have alterations in the inter-domain interface.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search