BBSRC Portfolio Analyser
Award details
Detection of singlet oxygen in tissues using time- and wavelength-correlated photon counting
Reference
B03962
Principal Investigator / Supervisor
Professor C Morgan
Co-Investigators /
Co-Supervisors
Professor Roger Hugh Bisby
,
Dr Anthony Corman
Institution
University of Salford
Department
Biomedical Sciences Research Institute
Funding type
Research
Value (£)
133,206
Status
Completed
Type
Research Grant
Start date
01/09/1995
End date
01/01/1998
Duration
28 months
Abstract
A novel instrument will be constructed for the imaging detection of singlet oxygen luminescence in cells and tissues. It will be used to map the spatial distribution of singlet oxygen formation due to reaction of ozone with cell constituents and that arising from photosensitised energy transfer to oxygen. High sensitivity will be achieved by acousto-optic spectral modulation coupled with the use of an up-conversion phosphor linked to an imaging photon detector. For dye-sensitised studies, the above scheme will be enhanced by temporal modulation of the exciting light source to exploit the long luminescence decay time of singlet oxygen. The instrument will permit investigation of the role and distribution of cellular constituents such as antioxidants which 'quench' singlet oxygen, decreasing the yield and lifetime of luminescence.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search