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Award details
Computer-aided design of proteins: testing design by expression and characterisation
Reference
B03891
Principal Investigator / Supervisor
Professor Alan Berry
Co-Investigators /
Co-Supervisors
Institution
University of Leeds
Department
Inst of Molecular & Cellular Biology
Funding type
Research
Value (£)
78,339
Status
Completed
Type
Research Grant
Start date
02/10/1995
End date
02/10/1997
Duration
24 months
Abstract
The de novo design of proteins is one of the outstanding unsolved problems of modern biochemistry and holds immense promise for science, medicine and industry. We have developed a general quantitative methodology to design proteins and have produced novel sequences to adopt the 3D fold of thioredoxin. An artificial gene will be constructed and the new protein will be overexpressed and purified. Structural characterisation will then be accomplished by a range of biophysical techniques including high field NMR spectroscopy. Site-directed and random mutagenesis in combination with biological selection will be used to increase our knowledge of the principles underlying the protein structures and to adapt our protein design algorithm.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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