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Towards a structure and mechanism for the hairpin ribozyme
Reference
B03825
Principal Investigator / Supervisor
Professor Jane Grasby
Co-Investigators /
Co-Supervisors
Institution
University of Sheffield
Department
Chemistry
Funding type
Research
Value (£)
151,798
Status
Completed
Type
Research Grant
Start date
01/06/1995
End date
01/12/1998
Duration
43 months
Abstract
The hairpin and hammerhead ribozymes are examples of a small catalytic RNases. We seek to probe structure/function relationships of the hairpin ribozyme in two ways. First, we shall use various specific RNases to identify single-stranded and base-paired regions of the ribozyme substrate complex. Secondly the functional groups of the essential nucleotides necessary for catalysis will be identified by synthesising mutant ribozymes. We shall measure the catalytic parameters of such modified ribozymes and evaluate their ability to bind the magnesium ion(s). It will thus be possible to differentiate between groups involved in Mg(II) binding and in hydrogen bonding. In separate mechanistic studies, the divalent metal ion requirements of the hammerhead and hairpin will be investigated and compared.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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