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Genome wide identification of proteins essential for cell cycle progression in Drosophila
Reference
APG16186
Principal Investigator / Supervisor
Professor David Glover
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Genetics
Funding type
Research
Value (£)
449,272
Status
Completed
Type
Research Grant
Start date
01/10/2002
End date
31/10/2006
Duration
49 months
Abstract
The potential function of approximately 13,600 Drosophila genes will be tested in cultured S2 cells by double stranded (ds) RNA interference. ds RNA will be generated from two groups of genes; one represented by a gridded array of approximately 6000 cDNA clones, and the second synthesised using PCR primers designed from the remaining approximately 8600 genes. Effects of ds RNA on the cell cycle progression of cultured S2 cells will be monitored by FACS analysis. ds RNAs eliciting a response will be analysed by immunostaining for markers of cell cycle progression. The identified gene products will be searched for functional motifs and their homology to genes in other organisms, particularly human. Functional studies will be initiated on a selection of human homologues.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
LINK: Applied Genomics (APG) [2000-2004]
Funding Scheme
X – not Funded via a specific Funding Scheme
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