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The molecular mechanisms by which PtdIns(345)P3 PtdIns(34)P2 and PtdIns3P signals are transduced

Reference	AF11409
Principal Investigator / Supervisor	Dr Phillip Hawkins
Co-Investigators / Co-Supervisors
Institution	Babraham Institute
Department	Babraham Institute Department
Funding type	Research
Value (£)	281,652
Status	Completed
Type	Fellowships
Start date	01/10/1998
End date	01/10/2003
Duration	60 months

Abstract

To identify the physiological function of a major new signal transduction pathway in mammalian cells: the hormone stimulated synthesis of 3-phosphorylated inositol lipids. The major problem to be dealt with was the development of specific inhibitors or dominant negative strategies with which to identify cell responses that were clearly P13K dependent. A distinct but very related component of the proposal was to identify the mechanisms by which different types of extracellular transmitters and/or cell-surface receptors could activate accumulation by which 3-phosphorylated lipids. The biggest long term target being to come to grips with the molecular mechanisms by which 3-phosphorylated lipids (and here we clearly focused on PtdIns(3,4,5)P3 as the most exciting candidate) could initiate intracellular signalling. To this end we proposed to drive forward collaborations with Chemistry groups aimed at synthesising these lipids and their stereoisomers (to act as controls) to enable cell- free assays to be reconstituted. We proposed, if time allowed, to use these lipids to test the hypothesis that PtdIns(3,4,5)P3 could activate specific protein kinases.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Fellowship - Advanced Fellowship (AF) [1995-1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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