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A coordinated approach to rapid fingerprinting and automatic sequencing of complex mixtures of oligosaccharides

Reference	ABY07396
Principal Investigator / Supervisor	Dr Raymond Dwek
Co-Investigators / Co-Supervisors	Professor Pauline Rudd
Institution	University of Oxford
Department	Biochemistry
Funding type	Research
Value (£)	238,767
Status	Completed
Type	Research Grant
Start date	01/06/1997
End date	01/06/2000
Duration	36 months

Abstract

Rapid, automated analysis is required to characterise the heterogeneous glycosylation of many biological samples. We demonstrate high resolution HPLC and capillary electrophoresis (CE) systems in which the elution positions of fluorescently labelled glycans are predictive, so that data generated from glycan pools can be interpreted directly from one analytical run. Structures were confirmed rapidly (2 days) by a novel strategy in which aliquots of the total glycan pool were simultaneously digested with arrays of immobilised exoglycosidases and analysed by HPLC. We aim to optimise conditions for analysing femtomoles of N-, O- and charged glycans and to provide standards for extending the data bases. A computer based data handling programme will be developed to generate conclusive analyses by combining data from native glycan pools and exoglycosidase digestions.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	LINK: Analytical Biotechnology (ABY) [1995-2000]
Funding Scheme	X – not Funded via a specific Funding Scheme

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