Award details

Novel approaches to 'hot start' gene amplification and process technologies for the polymerase chain reaction

Reference	ABC09830
Principal Investigator / Supervisor	Professor C Knowles
Co-Investigators / Co-Supervisors	Professor Mark Bailey, Professor Ian Jones
Institution	University of Oxford
Department	CEH Institute of Virology and Environmen
Funding type	Research
Value (£)	159,818
Status	Completed
Type	Research Grant
Start date	01/03/1999
End date	01/03/2002
Duration	36 months

Abstract

The polymerase chain reaction (PCR) is a powerful tool that has revolutionised the way modern molecular biology is carried out. However, despite recent advances in this field, problems persist including amplification of non-specific products in PCR and difficulty in rapid detection of those products. This project seeks to address the problems of this biocatalytic process, through use of a range of biochemical and molecular biology techniques. Generally, the approaches presented in this proposal include chemical and physical manipulation of the Thermus aquaticus DNA polymerase (Taq) used in PCR, biosorption/immobilisation of the enzyme, reversible Taq inhibition by novel peptides selected by phage display and by the use of thermoreversible buffers. Novel polymerase will also be screened for, and the PCR process will be studied to further our understanding of what methods can be used to detect PCR products.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	LINK: Applied Biocatalysis (ABC) [1993-1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

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