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Phosphoglycerate kinase or primer-recognition protein: another moonlighting protein
Reference
9912352
Principal Investigator / Supervisor
Professor John Bryant
Co-Investigators /
Co-Supervisors
Institution
University of Exeter
Department
Biosciences
Funding type
Research
Value (£)
37,638
Status
Completed
Type
Research Grant
Start date
01/09/1999
End date
01/09/2000
Duration
12 months
Abstract
We have purified and partially characterised a novel DNA-binding protein associated with DNA polymerase-alpha in pea. Its properties lead to the conclusion that it is a primer- recognition protein for lagging strand, DNA replication. It also appears to be identical to the glycolytic enzyme, phosphoglycerate kinase (it is a moonlighting protein). It is important to discover how the cell controls the two different activities and locations of the protein. This ROPA project has the specific objectives of construction of PHK-GFP fusions followed by transformation of Arabidopsis and tobacco to obtain preliminary data on the protein's subcellular location in different regions of the plant.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
ROPA 1999 (ROPA1999) [1999]
Funding Scheme
X – not Funded via a specific Funding Scheme
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