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Are SH2-binding motifs in neuronal nicotinic receptor subunits important for receptor trafficking and upregulation?
Reference
9912348
Principal Investigator / Supervisor
Ms Sue Wonnacott
Co-Investigators /
Co-Supervisors
Institution
University of Bath
Department
Biology and Biochemistry
Funding type
Research
Value (£)
92,784
Status
Completed
Type
Research Grant
Start date
01/03/2000
End date
01/08/2002
Duration
29 months
Abstract
A potential SH2-binding motif recognised by the SH2 domain of phosphoinositide- 3-kinase (PI-3 kinase) is conserved in several neuronal nicotinic receptor (nAChR) subunits. Its significance will be explored using mutagenesis and expression in stable cell lines, facilitating biochemical approaches. Given the role of PI-3 kinase in regulation of the endocytic pathway, the trafficking and upregulation of wildtype and mutated nAChR will be compared using confocal microscopy. This study will establish if nAChR are linked to lipid signalling pathways. If so, this proof of concept project will provide the first evidence for such an association between a ligand gated ion channel and PI-3 kinase.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
ROPA 1999 (ROPA1999) [1999]
Funding Scheme
X – not Funded via a specific Funding Scheme
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