Award details

Analysis and engineering of a surface bound protein in Lactococcus lactis

Reference	9912341
Principal Investigator / Supervisor	Professor Michael Gasson
Co-Investigators / Co-Supervisors	Dr Claire Shearman
Institution	Quadram Institute Bioscience
Department	Food Safety Science Division
Funding type	Research
Value (£)	89,100
Status	Completed
Type	Research Grant
Start date	01/06/2000
End date	01/06/2002
Duration	24 months

Abstract

CluA is a cell surface presented protein that causes cell aggregation and is essential for a high efficiency conjugation process. We will purify CluA both from Lactococcus and from E. coli strains expressing a variety of cluA genes. CluA antibodies will be raised and used to analyse CluA in different lactococcal constructs. Efficient and controlled expression of CluA on the lactococcal cell surface will be achieved. An analysis of the secondary structure of CluA will be undertaken so as to determine which regions are exposed on the cell surface. This will involve the construction of fusion proteins with staphyloccal nuclease and the introduction of tag peptides. Hybrid CluA proteins will be constructed using designs and expression systems chosen on the basis of the earlier studies. The major project objective will be to present biologically active and antigenic domains of the streptococcal protein SpaP that is involved in the development of dental caries.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	ROPA 1999 (ROPA1999) [1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

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