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New approaches to understanding phloem regulation: Direct measurement of specific mRNA in sieve elements

Reference9912327
Principal Investigator / Supervisor Dr Jeremy Pritchard
Co-Investigators /
Co-Supervisors
Institution University of Birmingham
DepartmentSch of Biosciences
Funding typeResearch
Value (£) 41,958
StatusCompleted
TypeResearch Grant
Start date 20/09/1999
End date 31/10/2000
Duration14 months

Abstract

We will measure the levels of specific mRNAs in phloem sap. This will be achieved by obtaining sieve tub sap by aphid stylectomy, reverse transcription to produce cDNA and amplification by PCR using sequence-specific primers. mRNA species will be selected on the basis of their proposed role in regulating sieve element content. Successful completion of the project will link cellular physiology with molecular approaches with considerable opportunity for exploitation in both pure and applied research.

Summary

unavailable
Committee Closed Committee - Plant & Microbial Sciences (PMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative ROPA 1999 (ROPA1999) [1999]
Funding SchemeX – not Funded via a specific Funding Scheme
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