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The expression of the Escherichia coli K5 polysaccharide in Saccharomyces cerevisiae
Reference
9810738
Principal Investigator / Supervisor
Professor Ian Roberts
Co-Investigators /
Co-Supervisors
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
41,005
Status
Completed
Type
Research Grant
Start date
01/04/1999
End date
01/04/2000
Duration
12 months
Abstract
The proposed work is to synthesise the Escherichia coli K5 polysaccharide on the cytoplasmic face of the endoplasmic reticulum in Saccharomyces cerevisiae. This will involve expressing the E. coli K5 kfiC and kfiD genes in S. cerevisiae. The KfiC protein is the glycosyltransferase which catalyses the synthesis of the K5 polysaccharide, whilst KfiD is a UDP-Glc dehydrogenase to provide the UDP-GlcA necessary for K5 biosynthesis. The expression of the K5 polysaccharide will be the first step in generating heparin-like molecules in S. cerevisiae and in the long term could lead to the engineering in S. cerevisiae of other polysaccharides of pharmacological importance.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
ROPA 1998 (ROPA1998) [1998]
Funding Scheme
X – not Funded via a specific Funding Scheme
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