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Selection of recombinant antibodies against GPI anchored proteins
Reference
9810700
Principal Investigator / Supervisor
Dr Mahendra Deonarain
Co-Investigators /
Co-Supervisors
Institution
Imperial College London
Department
Biological Sciences
Funding type
Research
Value (£)
78,280
Status
Completed
Type
Research Grant
Start date
01/06/1999
End date
01/12/2000
Duration
18 months
Abstract
Glycosylphosphatidylinositol (GPI)-linked glycoproteins are an important class of membrane-anchored proteins. We are developing a strategy to select for recombinant antibodies (from an immunised phage display library) which bind exclusively to the GPI-linked and not to the soluble form of the protein. These antibodies will be extremely useful in targeting cell surface proteins such as tumour-associated antigens (PLAP and CEA), where shed antigen is a problem, in the analysis of GPI-linked proteins such as cell-surface engineering and in the general study of the topology of membrane proteins. This approach is based upon whole cell positive and reverse sections.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
ROPA 1998 (ROPA1998) [1998]
Funding Scheme
X – not Funded via a specific Funding Scheme
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