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Modifications of heterologous proteins that may lead to inclusion body formation
Reference
9810659
Principal Investigator / Supervisor
Professor Lynne Roberts
Co-Investigators /
Co-Supervisors
Professor Michael Lord
Institution
University of Warwick
Department
Biological Sciences
Funding type
Research
Value (£)
111,011
Status
Completed
Type
Research Grant
Start date
01/03/1999
End date
01/07/2001
Duration
28 months
Abstract
A significant problem in the exploitation of recombinant DNA technology for heterologous protein production is the loss of activity associated with the trapping of polypeptides in inclusion body aggregates. Although many proteins can be recovered by denaturation and renaturation, this empirical process can be time consuming and inefficient. Clearly, a better understanding of the factors leading to protein aggregation in E. coli would be beneficial in many biotechnological applications. Based on our recent observations with recombinant pokeweed antiviral protein, we now propose to consider the effects of unexpected post-translational protein modification(s) as a possible contributory factor to inclusion formation.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
ROPA 1998 (ROPA1998) [1998]
Funding Scheme
X – not Funded via a specific Funding Scheme
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