BBSRC Portfolio Analyser
Award details
Identification of plant nuclear genes encoding mitochondrial proteins by genetic screening in yeast
Reference
9810657
Principal Investigator / Supervisor
Professor Chris Leaver
Co-Investigators /
Co-Supervisors
Institution
University of Oxford
Department
Plant Sciences
Funding type
Research
Value (£)
102,675
Status
Completed
Type
Research Grant
Start date
01/11/1998
End date
01/11/2000
Duration
24 months
Abstract
We propose to develop a novel genetic screen in yeast to isolate and characterise nuclear genes which encode mitochondrial proteins in plants. Arabidopsis thaliana cDNA libraries fused to a reporter gene essential for respiratory (i.e. mitochondrial) function but lacking a mitochondrial targeting sequence (MTS) will be transformed into a yeast strain deleted for the reporter gene. Selection of transformants able to grow on non-fermentable carbon sources will allow the recovery of a subset of cDNAs coding for plant proteins which contain an MTS. The novelty of this strategy is that random stretches of amino acids that could otherwise functionally replace a MTS will not be selected.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
ROPA 1998 (ROPA1998) [1998]
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search