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Mapping physical and biochemical interactions of multicomponent subcellular assemblies
Reference
9810651
Principal Investigator / Supervisor
Professor David Rice
Co-Investigators /
Co-Supervisors
Dr Iain Ashmore Murray
Institution
University of Sheffield
Department
Molecular Biology and Biotechnology
Funding type
Research
Value (£)
95,760
Status
Completed
Type
Research Grant
Start date
14/12/1999
End date
14/02/2002
Duration
26 months
Abstract
Histidine-tagged proteins can act as catalysts of oxidative protein crosslinking via a reaction which is limited to proteins which form specific intermolecular associations in solution. We propose to use the Saccharomyces cerevisiae spliceosome as a model system to demonstrate proof of principle in respect of the use of His-tagged proteins as tools to map intracellular protein:protein interactions. His-tagged spliceosomal proteins will be incorporated into yeast spliceosomes in vivo and subjected to oxidative cross-linking. Immunological analysis, using antisera raised against putative interacting (and control, non-interacting) splicing-associated proteins - previously identified by genetic (two-hybrid) methods - will be used to characterise the cross-linked products.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
ROPA 1998 (ROPA1998) [1998]
Funding Scheme
X – not Funded via a specific Funding Scheme
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