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Probing for apoplastic hydroxyl radicals (OH) in plant tissues
Reference
9810648
Principal Investigator / Supervisor
Professor Stephen Fry
Co-Investigators /
Co-Supervisors
Institution
University of Edinburgh
Department
Inst of Cell and Molecular Biology
Funding type
Research
Value (£)
38,157
Status
Completed
Type
Research Grant
Start date
01/01/1999
End date
01/01/2000
Duration
12 months
Abstract
In vitro, under conditions mimicking those found in plant cell walls, in vivo, hydroxyl radicals (OH) are readily generated and cause non-enzymic scission wall polysaccharides. Production of apoplastic OH is potentially a mechanism of wall loosening, important in cell expansion, fruit ripening etc. This project will test the hypothesis that apoplastic OH radicals are generated in vivo. A membrane-nonpermeant 'trap' molecule will be developed that in the presence of apoplastic OH liberates tritiated water, which can be quantified. Using this trapping' system, diverse plant tissues (believed to be undergoing wall loosening) will be surveyed for production of apoplastic OH.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
ROPA 1998 (ROPA1998) [1998]
Funding Scheme
X – not Funded via a specific Funding Scheme
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