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Role of cell-specific expression of Na+ transporters in salinity tolerance in Arabidopsis

Reference9810629
Principal Investigator / Supervisor Professor Mark Tester
Co-Investigators /
Co-Supervisors
Institution University of Cambridge
DepartmentPlant Sciences
Funding typeResearch
Value (£) 40,626
StatusCompleted
TypeResearch Grant
Start date 22/06/1999
End date 22/06/2000
Duration12 months

Abstract

The aim of the proposed research is to examine whether cell-specific expression of heterologous Na+ transporters will increase salinity tolerance in Arabidopsis. Genes from yeast for a plasma membrane Na+ ATPase and a tonoplast Na+/H+ antiporter will be inserted into Arabidopsis downstream of a GAL4-UAS. These plants will be crossed with lines in which GAL4 (and GFP) is expressed in specific cell types, to generate a series of lines with cell-specific expression of Na+ transporters. The intracellular location of the protein product will be monitored using YFP fusions to the Na+ transporters. This range of lines will be screened for Na+ tolerance relative to the control lines, preparatory for further physiological investigations.

Summary

unavailable
Committee Closed Committee - Plant & Microbial Sciences (PMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative ROPA 1998 (ROPA1998) [1998]
Funding SchemeX – not Funded via a specific Funding Scheme
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